Search results for: Puey Ounjai

Items from 1 to 12 out of 12 results

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Proteomic profiling of circulating β‐thalassaemia/haemoglobin E extra‐cellular vesicles reveals that association with immunoglobulin induces membrane vesiculation

Kunwadee Phongpao, Nuttanan Pholngam, Daranee Chokchaichamnankit, Khanita Nuamsee, more

British Journal of Haematology > 204 > 5 > 2025 - 2039

Splenectomised β‐thalassaemia/haemoglobin E (HbE) patients have increased levels of circulating microparticles or medium extra‐cellular vesicles (mEVs). The splenectomised mEVs play important roles in thromboembolic complications in patients since they can induce platelet activation and endothelial cell dysfunction. However, a comprehensive understanding of the mechanism of mEV generation in thalassaemia...

article

Glyphosate metabolism in Tetrahymena thermophila: A shotgun proteomic analysis approach

Abdul Manan, Sittiruk Roytrakul, Sawanya Charoenlappanit, Toemthip Poolpak, more

Environmental Toxicology > 38 > 4 > 867 - 882

Glyphosate is one of the most widely used herbicides in the world. However, because of its overuse and resistance to degradation, high levels of glyphosate residues in the environment are reported. Therefore, this study aimed to investigate the effects of glyphosate on proteomic aspects of Tetrahymena thermophila and their uses as bioindicators of freshwater ecosystem. First, an acute toxicity test...

article

Theoretical analysis of orientations and tautomerization of genistein in β-cyclodextrin

Chonnikan Hanpaibool, Tipsuda Chakcharoensap, Arifin, Yuh Hijikata, more

Journal of Molecular Liquids > 2018 > 265 > C > 16-23

Genistein is an isoflavone with promising pharmaceutical applications. However, its low water solubility interferes with its potency, and therefore cyclodextrins (CDs) have been considered as possible drug delivery system (DDS). To investigate the complexation mechanism of genistein in cyclodextrin, we employed molecular dynamics (MD) simulations based on classical potentials and the density-functional...

article

Oleic acid Enhances Dengue Virus But Not Dengue Virus-Like Particle Production from Mammalian Cells

Suwipa Ramphan, Sathiporn Suksathan, Nitwara Wikan, Puey Ounjai, more

Molecular Biotechnology > 2017 > 59 > 9-10 > 385-393

Despite the recent introduction of a commercial vaccine, the mosquito-transmitted dengue virus is still a worldwide public health problem. Based on the live attenuated vaccine strategy, the commercial vaccine has a less than optimal protective profile. Virus-like particles (VLPs) offer an attractive alternate vaccination strategy due to the effectively native presentation of epitopes in the absence...

article

C-terminal domain on the outer surface of the Macrobrachium rosenbergii nodavirus capsid is required for Sf9 cell binding and internalization

Monsicha Somrit, Atthaboon Watthammawut, Charoonroj Chotwiwatthanakun, Puey Ounjai, more

Virus Research > 2017 > 227 > C > 41-48

We have shown that Macrobrachium rosenbergii nodavirus (MrNV) was able to infect Sf9 cells and that MrNV virus-like particles (MrNV-VLPs) were capable nanocontainers for delivering nucleic acid-based materials. Here, we demonstrated that chymotryptic removal of a C-terminal peptide and its truncated variant (F344-MrNV-VLPs) exhibited a drastically reduced ability to interact and internalize into Sf9...

article

Inactivation of Clostridium difficile spores by microwave irradiation

Suvash Chandra Ojha, Surang Chankhamhaengdecha, Sombat Singhakaew, Puey Ounjai, more

Anaerobe > 2016 > 38 > C > 14-20

Spores are a potent agent for Clostridium difficile transmission. Therefore, factors inhibiting spores have been of continued interest. In the present study, we investigated the influence of microwave irradiation in addition to conductive heating for C. difficile spore inactivation in aqueous suspension. The spores of 15 C. difficile isolates from different host origins were exposed to conductive...

article

FMG1-B as a Eukaryotic S-Layer

Puey Ounjai, Kenneth H. Downing

Biophysical Journal > 2014 > 106 > 2 Supplement 1 > 359a

article

Architectural Insights into a Ciliary Partition

Puey Ounjai, Keunhwan D. Kim, Haichuan Liu, Ming Dong, more

Current Biology > 2013 > 23 > 4 > 339-344

Ciliary compartmentalization plays pivotal roles in ciliogenesis and in various signaling pathways. Here we describe a structure at the ciliary base that appears to have all the features required for compartmentalization and which we thus call the “ciliary partitioning system” (CPS). This complex consists of the terminal plate, which serves as a cytosolic “ciliary pore complex” (CPC), and a membrane...

article

High-Contrast In-Focus Imaging in Biological Cryo-EM

Robert M. Glaeser, Stefano Cabrini, Kenneth H. Downing, Bong-Gyoon Han, more

Biophysical Journal > 2012 > 102 > 3 Supplement 1 > 394a

article

Structural Basis Of RIG-I Auto-inhibition And RNA-induced Activation

Anirban Adhikari, Puey Ounjai, Qiu-Xing Jiang, Zhijian Chen

Biophysical Journal > 2009 > 96 > 3 Supplement 1 > 384a

article

Streptavidin crystals as nanostructured supports and image-calibration references for cryo-EM data collection

Liguo Wang, Puey Ounjai, Fred J. Sigworth

Journal of Structural Biology > 2008 > 164 > 2 > 190-198

For cryo-EM structural studies, we seek to image membrane proteins as single particles embedded in proteoliposomes. One technical difficulty has been the low density of liposomes that can be trapped in the ∼100nm ice layer that spans holes in the perforated carbon support film of EM grids. Inspired by the use of two-dimensional (2D) streptavidin crystals as an affinity surface for biotinylated DNA...

article

Two conformational states of the membrane-associated Bacillus thuringiensis Cry4Ba δ-endotoxin complex revealed by electron crystallography: Implications for toxin-pore formation

Puey Ounjai, Vinzenz M. Unger, Fred J. Sigworth, Chanan Angsuthanasombat

Biochemical and Biophysical Research Communications > 2007 > 361 > 4 > 890-895

The insecticidal nature of Cry δ-endotoxins produced by Bacillus thuringiensis is generally believed to be caused by their ability to form lytic pores in the midgut cell membrane of susceptible insect larvae. Here we have analyzed membrane-associated structures of the 65-kDa dipteran-active Cry4Ba toxin by electron crystallography. The membrane-associated toxin complex was crystallized in the presence...

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