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TILLING (Targeted Induced Local Lesions in Genomes; McCallum et al. 2000) is a reverse genetic tool for the identification of point mutations in genes of interest within EMS mutagenised populations (Till et al. 2003, Wienholds et al. 2003, Smits et al. 2004), facilitating the efficient screening of a considerable range of mutant alleles for their relation to gene function. TILLING employs the mismatch...
The following protocol provides a high throughput, low cost method of producing a superior DNA yield of high quality which is suitable for TILLING, map based cloning or any application which requires long term DNA storage. The protocol has been designed for DNA extraction from leaf material, preferably young leaf tissue should be used as this minimises samples being contaminated with polysaccharides...
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