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Introduction: We are investigating the use of electroporation in vivo to enhance transfection efficiency and improve wound healing with DNA plasmid expression vectors for growth factors.Methods: For electroporation (EP) transfection efficiency and wound healing we used luciferase and keratinocyte growth factor (KGF) DNA plasmid expression vectors respectively (gWIZ-Lux vector controlled by CMV promoter,...
Introduction: Correcting the wound healing deficit of diabetes is an unmet medical challenge. Diminished levels of important growth factors are found in wounds of diabetic individuals.Methods: We optimized in vivo electroporation (EP) parameters to six 100 microsecond pulses of 1750 volts/cm (ECM830, BTX Genetronics, San Diego CA). EP increased expression of Luciferase Plasmid DNA by 10 fold. We utilized...