Search results for: Eric Forest

Items from 1 to 20 out of 35 results

article

Cloning of the first human anti-JCPyV/VP1 neutralizing monoclonal antibody: Epitope definition and implications in risk stratification of patients under natalizumab therapy

Roberta Antonia Diotti, Nicasio Mancini, Nicola Clementi, Giuseppe Sautto, more

Antiviral Research > 2014 > 108 > Complete > 94-103

JC virus (JCPyV) has gained novel clinical importance as cause of progressive multifocal leukoencephalopathy (PML), a rare demyelinating disease recently associated to immunomodulatory drugs, such as natalizumab used in multiple sclerosis (MS) cases. Little is known about the mechanisms leading to PML, and this makes the need of PML risk stratification among natalizumab-treated patients very compelling...

article

Accessibility Changes within Diphtheria Toxin T Domain upon Membrane Penetration Probed by Hydrogen Exchange and Mass Spectrometry

Petr Man, Caroline Montagner, Heidi Vitrac, Daniel Kavan, more

Journal of Molecular Biology > 2011 > 414 > 1 > 123-134

The translocation domain of diphtheria toxin inserts in membrane and becomes functional when the pH inside endosomes is acid. At that stage, the domain is in a partially folded state; this prevents the use of high-resolution methods for the characterization of its functional structure. On that purpose, we report here the use of hydrogen/deuterium exchange experiments coupled to mass spectrometry....

article

Solid‐Phase Cross‐Linking (SPCL): A new tool for protein structure studies

David Paramelle, Christine Enjalbal, Muriel Amblard, Eric Forest, more

PROTEOMICS > 11 > 7 > 1277 - 1286

A wide range of chemical reagents are available to study the protein–protein interactions or protein structures. After reaction with such chemicals, covalently modified proteins are digested, resulting in shorter peptides that are analyzed by mass spectrometry (MS). Used especially when NMR of X‐ray data are lacking, this methodology requires the identification of modified species carrying relevant...

article

A new CZE method for profiling human serum albumin and its related forms to assess the quality of biopharmaceuticals

Youssef Alahmad, Nguyet Thuy Tran, Isabelle Le Potier, Eric Forest, more

ELECTROPHORESIS > 32 > 2 > 292 - 299

We present a new CZE method, which uses a polyethylene oxide‐coated capillary to separate native HSA from more than five of its structural variants. These variants include oxidized, truncated, and cysteinylated forms of HSA which can all be found in biopharmaceutical products. Both CE and MS confirmed the high degree of heterogeneity of HSA preparations. Recovery studies demonstrated that adsorption...

article

Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics

Martial Rey, Hynek Mrázek, Petr Pompach, Petr Novák, more

Analytical Chemistry > 2010 > 82 > 12 > 5107 - 5116

article

Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen/deuterium exchange measurements

Petr Man, Caroline Montagner, Heidi Vitrac, Daniel Kavan, more

The FEBS Journal > 277 > 3 > 653 - 662

The translocation domain (T domain) of diphtheria toxin adopts a partially folded state, the so‐called molten globule state, to become functional at acidic pH. We compared, using hydrogen/deuterium exchange experiments associated with MS, the structures of the T domain in its soluble folded state at neutral pH and in its functional molten globule state at acidic pH. In the native state, the α‐helices...

article

Investigating Alternative Acidic Proteases for H/D Exchange Coupled to Mass Spectrometry: Plasmepsin 2 but not Plasmepsin 4 Is Active Under Quenching Conditions

Julien Marcoux, Eric Thierry, Corinne Vivès, Luca Signor, more

Journal of the American Society for Mass Spectrometry > 2010 > 21 > 1 > 76-79

Structural studies of proteins by hydrogen/deuterium exchange coupled to mass spectrometry (DXMS) require the use of proteases working at acidic pH and low temperatures. The spatial resolution of this technique can be improved by combining several acidic proteases, each generating a set of different peptides. Three commercial aspartic proteases are used, namely, pepsin, and proteases XIII and XVIII...

article

Investigating alternative acidic proteases for H/D exchange coupled to mass spectrometry: Plasmepsin 2 but not plasmepsin 4 is active under quenching conditions

Julien Marcoux, Eric Thierry, Corinne Vivès, Luca Signor, more

Journal of The American Society for Mass Spectrometry > 2010 > 21 > 1 > 76-79

article

A Crescent-Shaped ALIX Dimer Targets ESCRT-III CHMP4 Filaments

Ricardo Pires, Bettina Hartlieb, Luca Signor, Guy Schoehn, more

Structure > 2009 > 17 > 6 > 843-856

ALIX recruits ESCRT-III CHMP4 and is involved in membrane remodeling during endosomal receptor sorting, budding of some enveloped viruses, and cytokinesis. We show that ALIX dimerizes via the middle domain (ALIX _-V ) in solution. Structural modeling based on small angle X-ray scattering (SAXS) data reveals an elongated crescent-shaped conformation for dimeric ALIX lacking the proline-rich...

article

Conformational changes in p47phox upon activation highlighted by mass spectrometry coupled to hydrogen/deuterium exchange and limited proteolysis

Julien Marcoux, Petr Man, Mathieu Castellan, Corinne Vivès, more

FEBS Letters > 2009 > 583 > 4 > 835-840

The neutrophil NADPH oxidase is an enzymatic complex involved in innate immunity. Phosphorylation of p47 ^phox promotes its translocation with p67 ^phox and p40 ^phox , followed by membrane interaction and assembly with flavocytochrome b ₅₅₈ into a functional complex. To characterise p47 ^phox conformational changes during activation, we used wild-type and the S303/304/328E...

article

Defining the Interacting Regions between Apomyoglobin and Lipid Membrane by Hydrogen/Deuterium Exchange Coupled to Mass Spectrometry

Petr Man, Caroline Montagner, Grégory Vernier, Bernard Dublet, more

Journal of Molecular Biology > 2007 > 368 > 2 > 464-472

Sperm whale myoglobin can be considered as the model protein of the globin family. The pH-dependence of the interactions of apomyoglobin with lipid bilayers shares some similarities with the behavior of pore-forming domains of bacterial toxins belonging also to the globin family. Two different states of apomyoglobin bound to a lipid bilayer have been characterized by using hydrogen/deuterium exchange...

article

Reversible Redox- and Zinc-Dependent Dimerization of the Escherichia coli Fur Protein

Benoît D'Autréaux, Ludovic Pecqueur, Anne Gonzalez de Peredo, Rutger E. M. Diederix, more

Biochemistry > 2007 > 46 > 5 > 1329 - 1342

article

Molecular Dissection of the Inhibitor Binding Pocket of Mitotic Kinesin Eg5 Reveals Mutants that Confer Resistance to Antimitotic Agents

Sébastien Brier, David Lemaire, Salvatore DeBonis, Eric Forest, more

Journal of Molecular Biology > 2006 > 360 > 2 > 360-376

The mitotic kinesin Eg5 plays an essential role in establishing the bipolar spindle. Recently, several antimitotic inhibitors have been shown to share a common binding region on Eg5. Considering the importance of Eg5 as a potential drug target for cancer chemotherapy it is essential to understand the molecular mechanism, by which these agents block Eg5 activity, and to determine the “key residues”...

article

Local dynamics measured by hydrogen/deuterium exchange and mass spectrometry of creatine kinase digested by two proteases

Hortense Mazon, Olivier Marcillat, Eric Forest, Christian Vial

Biochimie > 2005 > 87 > 12 > 1101-1110

Hydrogen/deuterium exchange coupled to mass spectrometry has been used to investigate the structure and dynamics of native dimeric cytosolic muscle creatine kinase. The protein was incubated in D ₂ O for various time. After H/D exchange and rapid quenching of the reaction, the partially deuterated protein was cleaved in parallel by two different proteases (pepsin or type XIII protease from...

article

Characterization of the DNA-binding site in the ferric uptake regulator protein from Escherichia coli by UV crosslinking and mass spectrometry

Ali Tiss, Olivier Barre, Isabelle Michaud-Soret, Eric Forest

FEBS Letters > 2005 > 579 > 25 > 5454-5460

Ferric uptake regulator protein (Fur) is activated by its cofactor iron to a state that binds to a specific DNA sequence called ‘Fur box’. Using mass spectrometry-based methods, we showed that Tyr 55 of Escherichia coli Fur, as well as the two thymines in positions 18 and 19 of the consensus Fur Box, are involved with binding. A conformational model of the Fur–DNA complex is proposed, in which DNA...

article

Major structural proteins of type 1 and type 3 Klebsiella fimbriae are effective protein carriers and immunogens in conjugates as revealed from their immunochemical characterization

Danuta Witkowska, Małgorzata Mieszała, Andrzej Gamian, Magdalena Staniszewska, more

FEMS Immunology and Medical Microbiology > 2005 > 45 > 2 > 221-230

Fimbriae are filamentous structures present on the cell surface of many bacteria, including genus Klebsiella. The use of fimbriae as protein carriers in conjugates may allow to formulate effective multivalent vaccines and suitable diagnostics. However, the evidences have been reported that fimbriae may enhance the inflammatory response. This prompted us to examine the degree of cytokine induction...

article

Identification of the Protein Binding Region of S -Trityl- l -cysteine, a New Potent Inhibitor of the Mitotic Kinesin Eg5 ^†

Sébastien Brier, David Lemaire, Salvatore DeBonis, Eric Forest, more

Biochemistry > 2004 > 43 > 41 > 13072 - 13082

article

Conformational Dynamics of the GdmHCl-Induced Molten Globule State of Creatine Kinase Monitored by Hydrogen Exchange and Mass Spectrometry

Hortense Mazon, Olivier Marcillat, Eric Forest, David L. Smith, more

Biochemistry > 2004 > 43 > 17 > 5045 - 5054

article

Mechanistic studies of the SufS-SufE cysteine desulfurase: evidence for sulfur transfer from SufS to SufE

Sandrine Ollagnier-de-Choudens, David Lascoux, Laurent Loiseau, Frédéric Barras, more

FEBS Letters > 2003 > 555 > 2 > 263-267

SufS is a cysteine desulfurase of the suf operon shown to be involved in iron-sulfur cluster biosynthesis under iron limitation and oxidative stress conditions. The enzyme catalyzes the conversion of L-cysteine to L-alanine and sulfide through the intermediate formation of a protein-bound cysteine persulfide in the active site. SufE, another component of the suf operon, has been previously shown to...

article

Changes in MM-CK Conformational Mobility upon Formation of the ADP−Mg ²⁺ −NO ₃ ^- −Creatine Transition State Analogue Complex As Detected by Hydrogen/Deuterium Exchange

Hortense Mazon, Olivier Marcillat, Eric Forest, Christian Vial

Biochemistry > 2003 > 42 > 46 > 13596 - 13604

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Search results for: Eric Forest

Cloning of the first human anti-JCPyV/VP1 neutralizing monoclonal antibody: Epitope definition and implications in risk stratification of patients under natalizumab therapy

Accessibility Changes within Diphtheria Toxin T Domain upon Membrane Penetration Probed by Hydrogen Exchange and Mass Spectrometry

Solid‐Phase Cross‐Linking (SPCL): A new tool for protein structure studies

A new CZE method for profiling human serum albumin and its related forms to assess the quality of biopharmaceuticals

Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics

Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen/deuterium exchange measurements

Investigating Alternative Acidic Proteases for H/D Exchange Coupled to Mass Spectrometry: Plasmepsin 2 but not Plasmepsin 4 Is Active Under Quenching Conditions

Investigating alternative acidic proteases for H/D exchange coupled to mass spectrometry: Plasmepsin 2 but not plasmepsin 4 is active under quenching conditions

A Crescent-Shaped ALIX Dimer Targets ESCRT-III CHMP4 Filaments

Conformational changes in p47phox upon activation highlighted by mass spectrometry coupled to hydrogen/deuterium exchange and limited proteolysis

Defining the Interacting Regions between Apomyoglobin and Lipid Membrane by Hydrogen/Deuterium Exchange Coupled to Mass Spectrometry

Reversible Redox- and Zinc-Dependent Dimerization of the Escherichia coli Fur Protein

Molecular Dissection of the Inhibitor Binding Pocket of Mitotic Kinesin Eg5 Reveals Mutants that Confer Resistance to Antimitotic Agents

Local dynamics measured by hydrogen/deuterium exchange and mass spectrometry of creatine kinase digested by two proteases

Characterization of the DNA-binding site in the ferric uptake regulator protein from Escherichia coli by UV crosslinking and mass spectrometry

Major structural proteins of type 1 and type 3 Klebsiella fimbriae are effective protein carriers and immunogens in conjugates as revealed from their immunochemical characterization

Identification of the Protein Binding Region of S -Trityl- l -cysteine, a New Potent Inhibitor of the Mitotic Kinesin Eg5 ^†

Conformational Dynamics of the GdmHCl-Induced Molten Globule State of Creatine Kinase Monitored by Hydrogen Exchange and Mass Spectrometry

Mechanistic studies of the SufS-SufE cysteine desulfurase: evidence for sulfur transfer from SufS to SufE

Changes in MM-CK Conformational Mobility upon Formation of the ADP−Mg ²⁺ −NO ₃ ^- −Creatine Transition State Analogue Complex As Detected by Hydrogen/Deuterium Exchange

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