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Polypeptides and antigens of cell membrane (CM) and flagellar (Fl) preparations of seven different stocks of Trypanosoma evansi were studied using sodium dodecyl sulphate polyacrylamide gel electrophoresis and by Western blotting. The stocks were collected from Bareilly, Bikaner, Hisar Karnal, Lucknow and Ludhiana localities of northern India and were isolated from either buffalo, equids or camel...
An enzyme-linked immunosorbent assay (ELISA) was employed for the detection ofTrypanosoma evansi antigens in serum samples of field cases of buffaloes and horses in northern India. In 323 naturally infected/suspected buffaloes, circulating antigenaemia was detected in 180 (55.72%), whereas parasitaemia by wet blood smear examination was found in 62 (19.19%) only. The antigen-ELISA was positive in...
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