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AimsThe objective of this study was to produce stable inclusions of chitinase ChiA74Δsp in Bacillus thuringiensis subsp. israelensis (Bti) and to assay its insecticidal activity against Aedes aegypti larvae.
Methods and ResultsBti was transformed with chiA74Δsp regulated by its own promoter or by the strong chimeric cytAp/STAB‐SD promoter system to generate two recombinant Bti strains. These recombinants...