Search results for: Jean-Pierre Simorre

Items from 1 to 20 out of 35 results

article

Induced conformational changes activate the peptidoglycan synthase PBP1B

Alexander J. F. Egan, Roberto Maya‐Martinez, Isabel Ayala, Catherine M. Bougault, more

Molecular Microbiology > 110 > 3 > 335 - 356

Bacteria surround their cytoplasmic membrane with an essential, stress‐bearing peptidoglycan (PG) layer consisting of glycan chains linked by short peptides into a mesh‐like structure. Growing and dividing cells expand their PG layer using inner‐membrane anchored PG synthases, including Penicillin‐binding proteins (PBPs), which participate in dynamic protein complexes to facilitate cell wall growth...

article

Cell division of Streptococcus pneumoniae: think positive!

Pierre Simon Garcia, Jean-Pierre Simorre, Céline Brochier-Armanet, Christophe Grangeasse

Current Opinion in Microbiology > 2016 > 34 > C > 18-23

Bacterial cell division is achieved by a dynamic protein complex called the divisome. The accurate placement of the divisome, and more specifically that of the tubulin-like protein FtsZ which forms the contractile Z-ring at mid-cell, is finely regulated by different mechanisms tailored to each bacterial class. To give rise to two viable daughter cells with the same genetic heritage and cell shape,...

article

Acyl acceptor recognition by Enterococcus faecium l,d‐transpeptidase Ldt_fm

Sébastien Triboulet, Catherine M. Bougault, Cédric Laguri, Jean‐Emmanuel Hugonnet, more

Molecular Microbiology > 98 > 1 > 90 - 100

In Mycobacterium tuberculosis and ampicillin‐resistant mutants of Enterococcus faecium, the classical target of β‐lactam antibiotics is bypassed by l,d‐transpeptidases that form unusual 3 → 3 peptidoglycan cross‐links. β‐lactams of the carbapenem class, such as ertapenem, are mimics of the acyl donor substrate and inactivate l,d‐transpeptidases by acylation of their catalytic cysteine. We have blocked...

article

On the Bacterial Cell Wall by Liquid State, Standard and DNP Solid State NMR

Jean-Pierre Simorre

Biophysical Journal > 2015 > 108 > 2 Supplement 1 > 43a-44a

article

Solution NMR assignment of LpoB, an outer-membrane anchored Penicillin-Binding Protein activator from Escherichia coli

Nicolas L. Jean, Catherine M. Bougault, Alexander J. F. Egan, Waldemar Vollmer, more

Biomolecular NMR Assignments > 2015 > 9 > 1 > 123-127

Bacteria surround their cytoplasmic membrane with the essential heteropolymer peptidoglycan (PG), which is made of glycan chains cross-linked by short peptides, to maintain osmotic stability and cell shape. PG is assembled from lipid II precursor by glycosyltransferase and transpeptidase reactions catalyzed by PG synthases, which are anchored to the cytoplasmic membrane and are controlled from...

article

Backbone and side-chain ¹ H, ¹³ C, and ¹⁵ N NMR assignments of the N-terminal domain of Escherichia coli LpoA

Nicolas L. Jean, Catherine Bougault, Adeline Derouaux, Gilles Callens, more

Biomolecular NMR Assignments > 2015 > 9 > 1 > 65-69

The peptidoglycan is a major component of the bacterial cell wall and is essential to maintain cellular integrity and cell shape. Penicillin-Binding Proteins (PBPs) catalyze the final biosynthetic steps of peptidoglycan synthesis from lipid II precursor and are the main targets of β-lactam antibiotics. The molecular details of peptidoglycan growth and its regulation are poorly understood. Presumably,...

article

Elongated Structure of the Outer-Membrane Activator of Peptidoglycan Synthesis LpoA: Implications for PBP1A Stimulation

Nicolas L. Jean, Catherine M. Bougault, Adam Lodge, Adeline Derouaux, more

Structure > 2014 > 22 > 7 > 1047-1054

The bacterial cell envelope contains the stress-bearing peptidoglycan layer, which is enlarged during cell growth and division by membrane-anchored synthases guided by cytoskeletal elements. In Escherichia coli, the major peptidoglycan synthase PBP1A requires stimulation by the outer-membrane-anchored lipoprotein LpoA. Whereas the C-terminal domain of LpoA interacts with PBP1A to stimulate its peptide...

article

Chemical shift perturbations induced by the acylation of Enterococcus faecium l,d-transpeptidase catalytic cysteine with ertapenem

Lauriane Lecoq, Catherine Bougault, Sébastien Triboulet, Vincent Dubée, more

Biomolecular NMR Assignments > 2014 > 8 > 2 > 339-343

Penicillin-binding proteins were long considered as the only peptidoglycan cross-linking enzymes and one of the main targets of β-lactam antibiotics. A new class of transpeptidases, the l,d-transpeptidases, has emerged in the last decade. In most Gram-negative and Gram-positive bacteria, these enzymes generally have nonessential roles in peptidoglycan synthesis. In some clostridiae and mycobacteria,...

article

Windowed R-PDLF recoupling: A flexible and reliable tool to characterize molecular dynamics

Axel Gansmüller, Jean-Pierre Simorre, Sabine Hediger

Journal of Magnetic Resonance > 2013 > 234 > Complete > 154-164

This work focuses on the improvement of the R-PDLF heteronuclear recoupling scheme, a method that allows quantification of molecular dynamics up to the microsecond timescale in heterogeneous materials. We show how the stability of the sequence towards rf-imperfections, one of the main sources of error of this technique, can be improved by the insertion of windows without irradiation into the basic...

article

Dynamics Induced by β-Lactam Antibiotics in the Active Site of Bacillus subtilis l,d-Transpeptidase

Lauriane Lecoq, Catherine Bougault, Jean-Emmanuel Hugonnet, Carole Veckerlé, more

Structure > 2012 > 20 > 5 > 850-861

β-lactams inhibit peptidoglycan polymerization by acting as suicide substrates of essential d,d-transpeptidases. Bypass of these enzymes by unrelated l,d-transpeptidases results in β-lactam resistance, although carbapenems remain unexpectedly active. To gain insight into carbapenem specificity of l,d-transpeptidases (Ldts), we solved the nuclear magnetic resonance (NMR) structures of apo and imipenem-acylated...

article

Isolation and analysis of cell wall components from Streptococcus pneumoniae

Nhat Khai Bui, Alice Eberhardt, Daniela Vollmer, Thomas Kern, more

Analytical Biochemistry > 2012 > 421 > 2 > 657-666

The complex and heterogeneous cell wall of the pathogenic bacterium Streptococcus pneumoniae is composed of peptidoglycan and a covalently attached wall teichoic acid. The net-like peptidoglycan is formed by glycan chains that are crosslinked by short peptides. We have developed a method to purify the glycan chains, and we show that they are longer than approximately 25 disaccharide units. From purified...

article

¹³ C-Labeled Heparan Sulfate Analogue as a Tool To Study Protein/Heparan Sulfate Interactions by NMR Spectroscopy: Application to the CXCL12α Chemokine

Cédric Laguri, Nicolas Sapay, Jean-Pierre Simorre, Bernhard Brutscher, more

Journal of the American Chemical Society > 2011 > 133 > 25 > 9642 - 9645

article

Dynamics Characterization of Fully Hydrated Bacterial Cell Walls by Solid-State NMR: Evidence for Cooperative Binding of Metal Ions

Thomas Kern, Mathilde Giffard, Sabine Hediger, Ana Amoroso, more

Journal of the American Chemical Society > 2010 > 132 > 31 > 10911 - 10919

article

Structural Basis of HIV-1 Tethering to Membranes by the BST-2/Tetherin Ectodomain

Andreas Hinz, Nolwenn Miguet, Ganesh Natrajan, Yoshiko Usami, more

Cell Host & Microbe > 2010 > 7 > 4 > 314-323

The restriction factor BST-2/tetherin contains two membrane anchors employed to retain some enveloped viruses, including HIV-1 tethered to the plasma membrane in the absence of virus-encoded antagonists. The 2.77 Å crystal structure of the BST-2/tetherin extracellular core presented here reveals a parallel 90 Å long disulfide-linked coiled-coil domain, while the complete extracellular domain forms...

article

Longitudinal-Relaxation-Enhanced NMR Experiments for the Study of Nucleic Acids in Solution

Jonathan Farjon, Jérôme Boisbouvier, Paul Schanda, Arthur Pardi, more

Journal of the American Chemical Society > 2009 > 131 > 24 > 8571 - 8577

article

Toward the Characterization of Peptidoglycan Structure and Protein−Peptidoglycan Interactions by Solid-State NMR Spectroscopy

Thomas Kern, Sabine Hediger, Patrick Müller, Cécile Giustini, more

Journal of the American Chemical Society > 2008 > 130 > 17 > 5618 - 5619

article

Solution Structure of NOD1 CARD and Mutational Analysis of its Interaction with the CARD of Downstream Kinase RICK

Florence Manon, Adrien Favier, Gabriel Núñez, Jean-Pierre Simorre, more

Journal of Molecular Biology > 2007 > 365 > 1 > 160-174

NOD1 is a cytosolic signalling host pattern-recognition receptor composed of a caspase-activating and recruitment domain (CARD), a nucleotide-binding and oligomerization domain (NOD) and leucine-rich repeats. It plays a crucial role in innate immunity by activating the NF-κB pathway via its downstream effector the kinase RICK (RIP2) following the recognition of a specific bacterial ligand. RICK is...

article

¹H, ¹³C and ¹⁵N resonance assignments of YajG, an Escherichia coli protein of unknown structure and function

Julien Boudet, Anne Chouquet, Aicha Chahboune, Cécile Giustini, more

Biomolecular NMR Assignments > 2007 > 1 > 1 > 89-91

The ampG gene codes for a permease required to uptake anhydro-muropeptides into bacterial cytoplasm. Located upstream in the same operon, is another 579-base-pair-long open reading frame encoding a putative lipoprotein YajG, whose nearly complete ¹H,¹³C,¹⁵N assignments are reported here.

article

Resolution-Enhanced Base-Type-Edited HCN Experiment for RNA

Hélène Van Melckebeke, Arthur Pardi, Jérôme Boisbouvier, Jean-Pierre Simorre, more

Journal of Biomolecular NMR > 2005 > 32 > 4 > 263-271

New base-type-edited transverse-relaxation optimized CT-HCN(C) experiments are presented that yield intra-base and sugar-to-base correlations for ¹³C−¹⁵N labeled RNA. High spectral resolution in the ¹³C and ¹⁵N dimensions is achieved by constant time (CT) frequency editing. A spectral editing filter applied during the CT ¹⁵N labeling period separates the correlation peaks arising from G/U and A/C...

article

Suppression of artifacts induced by homonuclear decoupling in amino-acid-type edited methyl 1H–13C correlation experiments

Hélène Van Melckebeke, Jean-Pierre Simorre, Bernhard Brutscher

Journal of Magnetic Resonance > 2004 > 170 > 2 > 199-205

A detailed theoretical and experimental analysis of the artifacts induced by homonuclear band-selective decoupling during CT frequency labeling is presented. The effects are discussed in the context of an amino-acid-type editing filter implemented in ¹ H– ¹³ C CT-HSQC experiments of methyl groups in proteins. It is shown that both Bloch–Siegert shifts and modulation sidebands are efficiently...

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Keywords

PEPTIDOGLYCAN (4)
NMR (3)
NMR RESONANCE ASSIGNMENT (3)
RESONANCE ASSIGNMENT (3)
TROSY (3)
ANTIBIOTIC RESISTANCE (2)
BACTERIAL CELL WALL BIOGENESIS (2)
HCN (2)
NUCLEIC ACIDS (2)
OUTER-MEMBRANE LIPOPROTEIN ACTIVATOR OF PBP (2)
RNA (2)
SOLID-STATE NMR (2)
SPECTRAL EDITING (2)
13C RELAXATION (1)
ESCHERICHIA COLI (1)
ADIABATIC PULSES (1)
AMINO-ACID-TYPE EDITING (1)
AMPG OPERON (1)
APOPTOSIS (1)
AUTOMATED ASSIGNMENT (1)
BAND-SELECTIVE DECOUPLING (1)
BASE-TYPE SELECTION (1)
BLOCH–SIEGERT SHIFTS (1)
C-C FILTER (1)
CARBAPENEM (1)
CARBON-13 (1)
CELL DIVISION COMPLEX (1)
CELL ELONGATION COMPLEX (1)
CELLBIO (1)
CHEMICAL SHIFT (1)
COLICIN TRANSLOCATION (1)
CONFORMATIONAL EXCHANGE (1)
CROSS CORRELATION (1)
CSA (1)
CYTOCHROME (1)
DIPOLAR COUPLING (1)
DIPOLAR RECOUPLING (1)
FOUR HELIX BUNDLE (1)
GLYCINE DECARBOXYLASE COMPLEX (1)
H-PROTEIN (1)
HADAMARD SPECTROSCOPY (1)
HETERONUCLEAR (1)
HETERONUCLEAR NMR ASSIGNMENT (1)
HOMONUCLEAR DECOUPLING (1)
INFLAMMATION (1)
L,D-TRANSPEPTIDASE (1)
LIPOATE-CONTAINING PROTEIN (1)
MAGIC-ANGLE SPINNING (1)
METHYL (1)
MICROBIO (1)
MULTIPLE QUANTUM COHERENCE (1)
MUROPEPTIDES (1)
NF-ΚB (1)
NMR ASSIGNMENT (1)
NMR SPECTROSCOPY (1)
NOD1/CARD4 (1)
NOE (1)
NOESY (1)
NUCLEAR MAGNETIC RESONANCE (1)
NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY (1)
PDLF (1)
PROTEIN NMR (1)
PROTEIN-DNA INTERACTION (1)
PROTEINS (1)
RELAXATION (1)
RESOLUTION ENHANCEMENT (1)
RICK/RIP2/CARDIAK (1)
SECONDARY STRUCTURE (1)
SIDEBANDS (1)
STREPTOCOCCUS PNEUMONIAE (1)
SYMMETRY-BASED SEQUENCE (1)
TEICHOIC ACID (1)
TOL-PAL SYSTEM (1)
TOLA PROTEIN (1)
TRIPLE RESONANCE (1)
TRIPLE RESONANCE NMR EXPERIMENTS (1)
WINGED HELIX PROTEIN (1)
YAJG (1)
Β-LACTAM (1)
Β-LACTAMASE REPRESSOR BLAI (1)
more

Data set

Elsevier (13)
ACS (10)
Springer (10)
Wiley (2)

Journal

Journal of Biomolecular NMR (6)
Journal of the American Chemical Society (6)
Biochemistry (4)
Biomolecular NMR Assignments (4)
FEBS Letters (3)
Journal of Magnetic Resonance (2)
Journal of Molecular Biology (2)
Molecular Microbiology (2)
Structure (2)
Analytical Biochemistry (1)
Biophysical Journal (1)
Cell Host & Microbe (1)
Current Opinion in Microbiology (1)
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INFONA - science communication portal

Search results for: Jean-Pierre Simorre

Induced conformational changes activate the peptidoglycan synthase PBP1B

Cell division of Streptococcus pneumoniae: think positive!

Acyl acceptor recognition by Enterococcus faecium l,d‐transpeptidase Ldt_fm

On the Bacterial Cell Wall by Liquid State, Standard and DNP Solid State NMR

Solution NMR assignment of LpoB, an outer-membrane anchored Penicillin-Binding Protein activator from Escherichia coli

Backbone and side-chain ¹ H, ¹³ C, and ¹⁵ N NMR assignments of the N-terminal domain of Escherichia coli LpoA

Elongated Structure of the Outer-Membrane Activator of Peptidoglycan Synthesis LpoA: Implications for PBP1A Stimulation

Chemical shift perturbations induced by the acylation of Enterococcus faecium l,d-transpeptidase catalytic cysteine with ertapenem

Windowed R-PDLF recoupling: A flexible and reliable tool to characterize molecular dynamics

Dynamics Induced by β-Lactam Antibiotics in the Active Site of Bacillus subtilis l,d-Transpeptidase

Isolation and analysis of cell wall components from Streptococcus pneumoniae

¹³ C-Labeled Heparan Sulfate Analogue as a Tool To Study Protein/Heparan Sulfate Interactions by NMR Spectroscopy: Application to the CXCL12α Chemokine

Dynamics Characterization of Fully Hydrated Bacterial Cell Walls by Solid-State NMR: Evidence for Cooperative Binding of Metal Ions

Structural Basis of HIV-1 Tethering to Membranes by the BST-2/Tetherin Ectodomain

Longitudinal-Relaxation-Enhanced NMR Experiments for the Study of Nucleic Acids in Solution

Toward the Characterization of Peptidoglycan Structure and Protein−Peptidoglycan Interactions by Solid-State NMR Spectroscopy

Solution Structure of NOD1 CARD and Mutational Analysis of its Interaction with the CARD of Downstream Kinase RICK

¹H, ¹³C and ¹⁵N resonance assignments of YajG, an Escherichia coli protein of unknown structure and function

Resolution-Enhanced Base-Type-Edited HCN Experiment for RNA

Suppression of artifacts induced by homonuclear decoupling in amino-acid-type edited methyl 1H–13C correlation experiments

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