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This paper reports a new class of biosensors based on composite nanomaterial thin film. The composite nanomaterial film is synthesized using a simple vacuum filtration method. Using composite nanomaterial film, we can not only simplify the positioning and integration fabrication process of nanoscale materials into a functional device, but also enhance the sensing surface area significantly, which...
In this paper, a simple and robust sensing assay platform for detection of biological materials is reported. The fluorescence based sensing platform benefits from the combined use of our novel dye-doped nanoparticle tags and nanoporous high surface area (~1,400 m2/g) films to achieve high intrinsic signal amplification. The dye-doped nanoparticles are synthesized from poly-methylsilsesquioxane (PMSSQ)...
A highly sensitive and stable L-Lactate sensor based on the synergic action of multi-wall carbon nanotube (MWCNTs) and ZnO nanoparticles has been developed. The unique sandwich-like layer structure (PDDA/LOD/ZnO/MWCNTs) provides a favorable microenvironment to keep the bioactivity of LOD and prevent enzyme molecule leakage. Therefore, the proposed L-Lactate biosensor exhibited good analytical performances...
Nano-materials show great electrocatalysis on redox reaction, e.g. metal nano-particles and carbon nano-tube. The electrocatalytic activity of semiconductor nano-particles, however, has not been reported although semiconductor was widely used as an electrode substrate for a variety of applications. In this work, we demonstrated a remarkable catalysis on electro-oxidation of ascorbic acid on indium...
Gold - poly(dimethylsiloxane) (Au - PDMS) nanocomposite freestanding films were prepared and used to study the binding of Human Serum Albumin (HSA) to polyclonal anti-HSA. A new method of synthesis was developed for sensing application when most of the gold nanoparticles have to be localized in the surface layer. The localized surface plasmon resonance band in gold nanoparticles is used to monitor...
A virus-gold nanoparticle (AuNP) composite film was prepared through the layer-by-layer assembly of cationic filamentous virus (M13 phage) particles (displaying a positively charged peptide on the major coat) and anionic spherical AuNPs. The surface plasmon resonance (SPR) for AuNPs in the virus-nanoparticle nanocomposite films was found to be sensitive to the environmental humidity. In air, both...
A novel optical biosensor with gold surface bound silver nanoparticles has been constructed for detection of bio-molecules like antigen, antibody protein and DNA plasmid, which exhibited distinct optical properties of localized surface plasmon resonance (LSPR). The silver colloidal nanoparticles were prepared by using sodium borohydride reduction of silver nitrate and characterized by using UV-vis...
This paper reports a rapid, simple and direct method for the synthesis of gold nanoparticles (AuNPs) in polypyrrole (PPy) film, which provides a biocompatible matrix for the strong adsorption of antibodies. The electrochemical synthesis of AuNPs/PPy composite on gold or carbon electrode occurs within 20 minutes, and does not involve addition of any nanoparticles or reducing agent. This method is compatible...
We are developing a novel reporter for proximity assays based on enhanced lanthanide luminescence promoted by metal nanoparticles. This method should provide high on/off ratios using photostable labels.
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